Journal: Arthritis Research & Therapy

Article Title: Circ-CAMTA1 regulated by Ca 2+ influx inhibited pyruvate carboxylase activity and modulate T cell function in patients with systemic lupus erythematosus

doi: 10.1186/s13075-024-03422-6

Figure Lengend Snippet: The expression levels of circ-CAMTA1, circ-ASH1L, and circ-ASAP1 in Jurkat cells after stimulation. Jurkat cells were stimulated with PMA 20 ng/mL + Ionomycin (Iono) 500 ng/mL for 24 h. ( A ) The expression levels of circ-CAMTA. ( B ) The expression levels of circ-ASH1L. ( C ) The expression levels of circ-ASAP1. Jurkat cells were stimulated with 1000 IU/mL recombinant human interferon-alpha (IFN-α) for 72 h. ( D ) The expression levels of circ-CAMTA1. ( E ) The expression levels of circ-ASH1L. ( F ) The expression levels of circ-ASAP1. Data was presented as mean ± SD. (ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: To analyzed circRNA expression levels in Jurkat cells after stimulation, cells were treated with phorbol 12-myristate 13-acetate (PMA; 20 ng/mL; Sigma-Aldrich) and ionomycin (500 ng/mL; Sigma-Aldrich, ) for 24 h. For analysis of circRNA expression in response to interferon-alpha (IFN-α), Jurkat cells were treated with 1000 IU/mL recombinant human IFN-α for 72 h. The IFN-alpha we used was purchased from Proteintech (Rosemont, IL, USA) and was produced in HEK293.

Techniques: Expressing, Recombinant